Efficacy of the two PCR kits for SARS-CoV-2 nucleic acid Detection

-Srilagna Sarkar, Amity University Kolkata

To compare the diagnostic efficacy of the two different reverse transcription-polymerase chain reaction (RT-PCR) test kits. Throat swab samples from 18 hospitalized patients who were clinically tested for COVID-19 and from 100 hospitalized patients who were COVID negative were taken.
SARS-CoV-2 nucleic acid was detected in throat swab samples of the Sansure PCR kit and Sanghai BioGerm PCR kit respectively.

The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and the kappa value were analyzed and three parallel tests of the three weakly positive samples were performed to analyse the respective values.

For all of the three parallel tests in all of the three samples, the coefficient of variation value of the BioGerm PCR kit was smaller for both ORF1ab and N gene compared to Sansure RT PCR kits. Detecting that the efficacy of the BioGerm PCR kit for SARS-CoV-2 nucleic acid detection was relatively higher than the Sansure PCR kit.

Source: https://onlinelibrary.wiley.com/doi/10.1002/jcla.23554

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