Camelia Bhattacharyya, Amity University Kolkata
With researches going on in the field of technology, something most important would now be the availability of a device that is capable of making a process faster and simpler. Biological studies are the ones needing such a system most urgently. A lot of data has to be collected each day and tested for proving any particular phenomenon or abnormality or difference with cent percent surety. Thus, there’s a need for something which can test on several samples simultaneously and with accuracy. Such an instrument has now arrived in the field of biological testing and it’s a very new type of microscopy known as the 2.5D microscopy.
The throughput of a microscope is what makes it faster and more efficient on a large number of samples. Creating a technique to increase the throughput was something of utmost importance. 2.5D microscopy does exactly that. In this technique, fluorescent light is focused on a plane of 2*2 (mm)2. Different in-focus information gets stored from different positions of the plane through different image capture. These images are then engineered into a single volumetric frame of frequency >30 Hz. These images go through less bleaching and thus provide more clarity and ease of study. The depth of the image can be manipulated as required by the researcher. This process is thus faster with high-resolution imaging focusing on bringing volumetric photographic information into one 2D frame.
This process can thus be used to find out quantitative subcellular properties in just about 10 minutes within which time, the technology provides all the information. Now the study and analysis of this information depend solely on the researcher who can now easily do it in less time and with more information. This technique thus needs to be applied for effective results in less time in order to take biological sciences a step forward.
Also read: The Kauri Tree: Untold story of the Earth being “upside-down”
Source: Ren J, Han KY., (2021). “2.5D Microscopy: Fast, High-Throughput Imaging via Volumetric Projection for Quantitative Subcellular Analysis.” ACS Photonics. doi:10.1021/acsphotonics.1c00012
One thought on “2.5D Microscopy: Volumetric Projection for Quantitative Subcellular Analysis”