Shrestha Dutta, Amity University Kolkata
The Tumor microenvironment impacts pancreatic Tumor advancement and progression. Pancreatic Tumor organoid model systems that include stromal cells can be utilized to contemplate the interactions between malignant cells and the supporting stromal cells. The advantage of these systems and optical redox imaging is to show that pancreatic stellate cells (PSCs) assume a more oxidized redox state than malignant cells and may participate in redox interaction with cancer cells to support proliferation. This interaction could help pancreatic malignant cells to accomplish oxidation reactions despite restricted access to oxygen as an electron acceptor, and direct contact between cancer cells and PSCs is important to increase the effect. Optical redox imaging has been recently used to study the metabolic interactions between HeLa cells and human skin fibroblasts.
The researches show that the fibroblasts were more oxidized in contrast with malignant cells in 2D monoculture. Optical redox imaging additionally uncovered a more oxidized state for the human MIA PaCa-2 PDAC cell line when co-cultured in 3D with patient-derived CAFs compared to monoculture conditions however didn’t evaluate the redox state of the CAFs. However, these examinations further support a theory where interactions between malignant cells and stromal cell can promote a more oxidized state in cancer cells.
Access to electron acceptors assist oxidized biomass synthesis and can be limiting for malignant cell growth. Non-transformed cells in tumours can help malignant cells reduce metabolic limitations, especially in pancreatic malignancy, where pancreatic stellate cells (PSCs) advance malignant growth cell expansion and tumour development. It is not known whether PSCs affect the redox condition of malignant cells. By taking the advantage of endogenous fluorescence properties of diminished nicotinamide adenine dinucleotide cofactors and oxidized flavin adenine dinucleotide, we utilize optical imaging to evaluate the redox condition of pancreatic malignant growth cells and PSCs and find that the redox condition of malignant cells is more decreased while the redox condition of PSCs is more oxidized.
Direct interactions among PSCs and malignant cells advance a more oxidized state in cancer cells, proposing that metabolic interactions between malignant cells and PSCs is a mechanism to combat the redox limitations of cell growth in pancreatic cancer.
Also read: PHOSPHO1 AS A POTENTIAL TREATMENT FOR OBESITY AND DIABETES?
Sources:
Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumours: Rupsa Datta, Allison NLau, Sharanya Sivanand, Logan Florek, Jeffrey Wyckoff, Melissa C. Skala, Matthew G. Vander Heiden; doi: https://doi.org/10.1101/2020.10.20.347658
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