Team bioXone
The regulatory effects of long non-coding RNAs (lncRNAs) in a range of developmental processes and diseases have generated tremendous attention from the scientific community worldwide. Although the exact knowledge of their action is limited, a small number of well-studied lncRNAs have provided clues about their biological functions and mechanisms.
A well-studied novel lncRNA, known as GAS8-AS1 is found to play an essential regulatory role in Papillary thyroid cancer (PTC cells). The roles and mechanism of GAS8-AS1 on the miR-187-3p/ATG axis and miR-1343-3p/ATG7 axis in PTC cells were evaluated using bioinformatics analysis, a luciferase reporter assay, cell counting Kit-8 assay, immunohistochemistry analysis, transmission electron microscopy, and immunofluorescence. The analysis revealed that patients with PTC showed low GAS8-AS1 expression. This is associated with high tumor-node-metastasis stage and lymph node metastasis. In-vitro autophagy is promoted and PTC cell proliferation is inhibited by GAS8 -AS1, while in-vivo tumorigenesis is promoted by GAS8-AS1. GAS8-AS1 promoted autophagy of PTC cells is up-regulated by binding of the ATF2 transcription factor to the GAS8 -AS1 promoter. Autophagy of PTC cells is regulated by the up-regulation of ATG5 and ATG7 by GAS8 -AS1.
Thus this novel mechanistic approach of GAS8-AS1 makes it a potential prognostic biomarker and a therapeutic target for PTC.
Source:
Yuan Qin, Sun Wei, Wang Zhihong, Dong Wenwu, He Liang, Zhang Ting, Shao Liang, Zhang Hao, ATF2-
induced lncRNA GAS8-AS1 expression promotes autophagy of thyroid cancer cells by targeting the miR-
187-3p/ATG5 and miR-1343-3p/ATG7 axes(2020);Molecular Therapy, Nucleic Acid.
https://doi.org/10.1016/j.omtn.2020.09.022
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