-Ruchita Karmakar, Amity University Kolkata
COVID-19 has been a major issue worldwide. But the traces of COVID-19 can be detected by a prompt diagnosis, isolation of the patient, and a contract tracing. Molecular tests serve as a potential way out for the detection of COVID-19 among the patients. These tests have been conducted in many central laboratories, with a delay in treatment and control decisions.
CODA (CRISPR Optical Detection of Anisotropy) system is developed recently for the successful detection and a point-of-care for early diagnosis of COVID-19. This particular method has merged isothermal nucleic acid amplification, CRISPR/Cas12a activation, and the inception of signal in the single assay thereby excluding the extra manual steps that are involved. Signal detection is particularly based upon the ratiometric measures of fluorescent anisotropy which granted CODA to accomplish signal-to-noise ratio.
CODA is designed in such a manner that it helps in the detection of N1 and N2 genes of SARS-CoV-2 RNA. An LED-generated blue light passed like a linear polarizer through the samples or the specimens of nasal or oral swabs. After the blue light is passed the sample emitted green light which is oscillated in the intensity at the exact modulation frequency with the polarization components that directly depend on the molecular weight of the FA probes. Two main detectors captured both the parallel and perpendicular polarization components. Each of the detectors contained a photodiode and a convex lens. Extra polarizer and a bandpass filter were used that helped in rejecting the source and the undesirable polarization angles. Thus detecting the N1 and N2 genes of the SARS-CoV-2 RNA virus.
Thus CODA helps in the detection of COVID-19 with a low – noise, high optimal capability, and a controlled temperature.
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